Enhanced Bioconversion of Methane to Biodiesel by Methylosarcina sp. LC-4

The conversion of methane into liquid biofuels using methane-consuming bacteria, known as methanotrophs, contributes to sustainable development, it mitigates the problem climate change caused by greenhouse gases and aids in producing cleaner renewable energy. In present research, an efficient methanotroph, Methylosarcina sp. LC-4, was studied a prospective organism for biodiesel production methane. uptake rate enhanced 1.6 times 2.35 supplementing LC-4 with micronutrients, such copper tungstate, respectively. This unique ability isolated enables deployment methanotrophs-based processes various industrial applications. A Plackett–Burman statistical (PBD) design used quantify role micronutrients other media components nitrate minimal salt (NMS) biomass fatty acid methyl esters (FAME) yields. Nitrate, phosphate, tungstate had positive effect, whereas copper, magnesium, salinity negative effect. modified NMS media, formulated according results from PBD analysis, increased FAME yield (mg/L) 85.7%, content 13 ± 1% (w/w) among highest reported methanotrophs. obtained consisted majorly (~90%) C14–C18 saturated monounsaturated acids, making suitable use biodiesel.